Presentazione

da "www.sciencedaily.com", Oct. 16, 2009

By directly manipulating the activity of individual neurons, scientists have given flies memories of a bad experience they never really had, according to a report in the October 16th issue of the journal Cell, a Cell Press publication.

"Flies have the ability to learn, but the circuits that instruct memory formation were unknown," said Gero Miesenböck of the University of Oxford. "We were able to pin the essential component down to 12 cells. It's really remarkable resolution." Those dozen cells are sufficient to manage what is a difficult cognitive problem: learning to associate a particular odor with something bad, like an electric shock. In essence, these cells create memories that the fly then uses to avoid that odor.

To pinpoint the exact neurons responsible for this memory among thousands in the fly brain, the researchers used a clever technique they developed, called optogenetics, in which a simple flash of light is used to release caged-molecules present in selective neurons that then stimulate the activity of those neurons. An analogous situation, says Miesenböck, is if you wanted to send a message only to certain inhabitants of a city, you would give those you wanted to reach a radio tuned to the right frequency and send the message publicly, over the airwaves.

Miesenböck said his team made some educated guesses about the parts of the brain that would be important for the flies' learning task. From there, they were able to narrow it down through experimentation to the 12-neuron brain circuit. Remarkably, stimulating just these neurons gives the flies a memory of an unpleasant event that never occurred.

"We like to take seemingly lofty psychological phenomena and reduce them to mechanics, to see for example how the intelligence needed to adapt to a changing environment can be reduced to physical interactions between cells and molecules," he said. "The question is: how do you get intelligence from parts that are unintelligent?"

Using their approach to "write directly to memory," scientists can now obtain a level of evidence about brain function that was impossible before, Miesenböck emphasized. He notes that neuroscience for a long time depended primarily on recording neural activity and attempting to correlate it to perceptions, actions, and cognition. "It's more powerful to seize control of the relevant brain circuits and produce these states directly," he says.

Miesenböck adds that the simple brain of a fly likely can tell us much about how more complex brains work. "As a general rule, biology tends to be conservative," he said. "It's rare that evolution 'invents' the same process several times." And, he says, even simple organisms may turn out to have a "surprisingly rich mental life."

The researchers include Adam Claridge-Chang, University of Oxford, Oxford, UK; Robert D. Roorda, University of Oxford, Oxford, UK; Eleftheria Vrontou, University of Oxford, Oxford, UK; Lucas Sjulson, University of Oxford, Oxford, UK; Haiyan Li, University of Virginia, Charlottesville, VA; Jay Hirsh, University of Virginia, Charlottesville, VA; and Gero Miesenbock, University of Oxford, Oxford, UK.